A novel thermophilic chitinase directly mined from the marine metagenome using the deep learning tool Preoptem
نویسندگان
چکیده
Abstract Chitin is abundant in nature and its degradation products are highly valuable for numerous applications. Thermophilic chitinases increasingly appreciated their capacity to biodegrade chitin at high temperatures prolonged enzyme stability. Here, using deep learning approaches, we developed a prediction tool, Preoptem, screen thermophilic proteins. A novel chitinase, Chi304, was mined directly from the marine metagenome. Chi304 showed maximum activity 85 ℃, T m reached 89.65 ± 0.22℃, exhibited excellent thermal stability 80 90 °C. had both endo- exo-chitinase activities, (GlcNAc) 2 main hydrolysis product of chitin-related substrates. The yields colloidal 97% within min, 20% over 4 days reaction with crude powder. This study thus provides method mine chitinase efficient biodegradation. Graphical
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ژورنال
عنوان ژورنال: Bioresources and Bioprocessing
سال: 2022
ISSN: ['2197-4365']
DOI: https://doi.org/10.1186/s40643-022-00543-1